Quantoom Biosciences’ Post

Beyond Viability: Advancing CHO Cell Culture Process Strategies to Modulate Host Cell Protein Levels While purification strategies usually offer substantial HCP clearance, certain problematic HCPs, particularly lipases, pose significant challenges. This study investigates the accumulation of various problematic HCPs in CHO cell culture using transcriptomics, revealing correlations between cell culture parameters and HCP activity. Contrary to conventional assumptions, viability alone does not reliably predict HCP levels, with factors such as clone selection, host cell line choice, and media feed type significantly influencing HCP accumulation. Leveraging transcriptomics-based approaches, we demonstrate the potential of upstream process control strategies to mitigate HCP presence and improve biologic product quality. Our findings underscore the importance of considering diverse cell culture parameters in bioprocess optimization to ensure product stability and quality. https://lnkd.in/g27Z3pc3 #aspenalert #biotech #bioprocess

Accelerating Cell Culture Media Development Using Bayesian Optimization-Based Iterative Experimental Design Here, we have applied a Bayesian Optimization-based iterative framework for experimental design to accelerate cell culture media development for two applications. First, we show this approach yields new compositions of media with cytokine supplementation to maintain the viability and distribution of PBMCs in culture. Second, we applied this framework to optimize the production of three recombinant proteins in K.phaffii cultivations. For both applications, we identified conditions with improved outcomes compared to the initial standard media using 3 to 30 times fewer experiments than other methods such as the Design of Experiments. Subsequently, we also demonstrated the extensibility of our approach to efficiently account for additional design factors through transfer learning. https://lnkd.in/g9c_7ZxK #aspenalert #biotech #bioprocess

📊 Deformulation Assays for #CRISPR LNPs!?! Best for last? Our final Oligos, Oligos, Oligos! webinar will be held Nov 21st at 9 am EST / 15:00 CST. I know many industry teammates were just at the International mRNA Health Conference and are probably overloaded with new info, but you won’t want to miss this! Big thanks to all of our featured speakers in this November’s webinar series. I’ll admit I have a favorite; we saved the best for last! What are you to do as an analytical scientist working on a new multi cargo #LNP? You’re handed a formulated particle and need to start qualifying new methods for component concentrations, their integrity and more… Acuitas Therapeutics, Inc., known for breakthroughs in synthetic lipids and novel LNP formulations, has been working with us on assays of just this sort. We have a choose-your-own-adventure scenario to present: choose a novel size exclusion or rapid IP-RP method with some important method robustness tweaks. These are brilliant experimentation stories from 2 talented scientists: Mateusz Imiołek and Razvan Cojocaru! 🗓️ Register here: https://lnkd.in/e2CCvDgV #GeneTherapy #CellTherapy #Biotechnology #LifeatWaters #AnalyticalChemistry Waters Corporation ————————— I’m Matt Lauber - an analytical biochemist who loves separating biomolecules, detecting masses, and all things biotechnology! ☑️ Follow & subscribe 🔔

https://lnkd.in/g-XxS7GN A Japanese research team has developed a drug that uses RNA to Target the USAG-1 protein to help regrow teeth! #ToothRegrowth #USAG1 #RNAtherapy #DentalInnovation #RegenerativeMedicine #ToothRegeneration #Biotechnology #DentalResearch #MedicalBreakthrough #InnovativeDentistry #FutureOfDentistry #KyotoUniversity #JapaneseResearch #OralHealth #StemCellTherapy Corey Stay AdapTatum

🚀 Exciting News: Introducing a new high-performance lyophilized product from New England Biolabs LyoSciences supporting molecular diagnostic developers! 🧬   Join us in revolutionizing nucleic acid amplification with our latest innovation: LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG)! https://lnkd.in/edcDg72A   Key Features: 🌿 Lyophilized format for ambient shipping and storage 🧬 One-step solution for Loop-Mediated Isothermal Amplification of DNA and RNA targets ⛔️ Thermolabile UDG and dUTP included to reduce carryover contamination 🌈 Includes fluorescent dye for real-time fluorescence measurement 🔥 WarmStart technology inhibits enzyme activity at room temperature   Whether you're in research, diagnostics, or biotech, our LyoPrime WarmStart fLAMP Mix offers unparalleled convenience and reliability. With a shelf life of 2 years at room temperature, you can trust in its performance whenever you need it.   Join the webinar on Lyophilizing Reagents to learn more about this groundbreaking technology and our expertise: https://lnkd.in/eTn4ZcEw   Let's amplify progress together! 💡 #NEB #NEBLS #Lyo #Lyophilisation #molecularbiology #DNA #RNA #diagnostics #biotech #LAMP

Heard the term #spatialtranscriptomics but not sure what it means? 🤔 Our brief overview explains exactly what it means, how it works and how spatial transcriptomics is used in drug discovery and development. Read it now by clicking the link in our comment below. #drugdiscovery #drugdevelopment #bioinformatics

Our expertise in engineering cell-based assays to monitor protein degradation is industry-leading. Our cell line engineering team creates dTag cell lines for reliable target validation, Hibit cell lines for real-time monitoring of protein degradation, or HaloTag fusion proteins for dynamic degradation tracking. Explore the breadth of our tools for the cutting-edge discovery of protein degraders with precision at https://lnkd.in/dghrFMHX Our scientists engineer new cell-based assays to answer the most pressing questions in protein degrader discovery: -> Target validation > Target degradation tracking > Real-time monitoring of target degradation Want to learn more? Download our brochure at https://lnkd.in/diCvwZty #targetedproteindegradation #drugdiscovery #protac #proteindegradation #TPD #proteindegraders

Bioprocess Feeding Optimization Through In-Silico Dynamic Experiments and Hybrid Digital Models - A Proof of Concept In this work we suggest a methodology to perform the feeding schedule optimization of mammalian cell cultures by virtualizing part of the experimental campaign on a hybrid digital model of the process, which accelerates the experimentation and reduces the experimental burden. The proposed methodology couples Design of Dynamic Experiments (DoDE) with a hybrid semi-parametric digital model. In particular, DoDE is used to design optimal experiments with time-varying factors’ profiles whose experimental data are then utilized to train the hybrid model which is able to identify the optimal time profiles of glucose and glutamine maximizing the antibody titer in the culture despite the limited number of experiments performed on the process. https://lnkd.in/ghabD9Az #aspenalert #biotech #bioprocess

Checkout the PolyJet™ In Vitro DNA Transfection Reagent by SignaGen Laboratories — available now at FroggaBio! Formulated with proprietary polymer synthesis technology for efficient and reproducible transfection in mammalian cells. 🐸🧬🔬 LEARN MORE: https://hubs.li/Q02PKZLN0 The PolyJet™ reagent ensures rapid and complete degradation after endocytosis, minimizing cytotoxicity. It offers high titers of virus production and is suitable for both single and multi-DNA transfection. Explore its broad transfection spectrum today! 🌟 #PolyJet #DNAtransfection #Biotechnology #MammalianCells #Science #Research #CellCulture #Genetics #TransfectionEfficiency #LabLife #Innovation #FroggaBio #Biodegradable #RecombinantProtein #STEM #PolymerSynthesis #TransfectionProtocol #CellBiology #LifeSciences #LabResearch #PolyJetReagent #SignaGen #LabTech #TransfectionReagent #ScientificDiscovery #Bioengineering #CellularResearch

From the basics of cell culture to advanced 3D models and therapeutic applications, the quality and consistency of media and reagents are key! High-quality components ensure reliable and reproducible results, vital for research and clinical progress. By using specialized growth factors, cytokines, and cutting-edge live-cell analysis technologies like the Incucyte® system, researchers can optimize cell culture conditions, enhance data accuracy, and drive innovative treatments forward. Our latest eBook highlights the importance of meticulous cell culture practices, showing how superior media and technologies can lead to groundbreaking advancements in research and therapy. 📚✨ Download now: https://ow.ly/LvUl50Tv7i2 #CellCulture #CellAnalyis #3DCellCulture #Organoids #Spheroids #Biotechnology