Aspen Alert’s Post

🔍Very interesting article in the September edition of GEN (Genetic Engineering & Biotechnology News, pages 22-24) comparing scale-out vs. scale-up: https://lnkd.in/eEUUQkHD For RNA production, scaling out provides key advantages: 🤸♂️ Flexibility in production scale ✨ Ease of operation 💰 CAPEX savings 🚀 Improved production performance   At Quantoom Biosciences, our RNA production operates in a repeat batch mode: 20mL IVT. All IVT reactions in Ntensify™ midi perform uniformly and with minimized shear forces. Plus, purchasing disposables in bulk helps reduce costs, as they are suitable across all production scales. By choosing scale-out, RNA production is efficient, adaptable, and cost-effective! #RNA #ScaleOut #Ntensify

Accelerating Cell Culture Media Development Using Bayesian Optimization-Based Iterative Experimental Design Here, we have applied a Bayesian Optimization-based iterative framework for experimental design to accelerate cell culture media development for two applications. First, we show this approach yields new compositions of media with cytokine supplementation to maintain the viability and distribution of PBMCs in culture. Second, we applied this framework to optimize the production of three recombinant proteins in K.phaffii cultivations. For both applications, we identified conditions with improved outcomes compared to the initial standard media using 3 to 30 times fewer experiments than other methods such as the Design of Experiments. Subsequently, we also demonstrated the extensibility of our approach to efficiently account for additional design factors through transfer learning. https://lnkd.in/g9c_7ZxK #aspenalert #biotech #bioprocess

Methods of Improving Protein Titer in Cell Culture A U.S. Patent for "Methods of Improving Protein Titer in Cell Culture" was awarded to Regeneron on July 9th, 2024. The present invention are methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. https://lnkd.in/gNEkZHwR #aspenalert #biotech #bioprocess

🚀 Are you looking to detect and quantify extracellular vesicles (EVs) with precision? 🔥Do you need high-quality labeled #antibodies for CD9, CD63, and CD81 detection in your PICO experiments? 🔬 Look no further—our Labeled EV Antibodies (EV-LABs) are here for you. Our EV-LABs are specifically selected and tested high-quality antibodies labeled with PICOglue Labels. Analyze your EV markers seamlessly with PICO technology using our EV-LABs. Eager to see how EV-LABs can enhance your EV analysis? Order them today 👉 https://lnkd.in/eiCict-Q 👈 📞 For any further information, feel free to write to us at sales@actome.de. #nextgenerationdiscovery #proteinquantification #actome #research #interactomics #antibodies #biotechnology #proteinanalysis #innovation #dPCR #interaction #dnasequencing #extracellularvesicles

Lipid Nanoparticles (LNPs) are transforming the landscape of CRISPR delivery systems, offering a powerful alternative to electroporation and lipo transfection. In our latest blog post, we dive into why LNPs are emerging as a preferred choice of delivery for CRISPR-based therapy development. 🔗 Learn more: https://lnkd.in/eAV2UVEy #CRISPR #GeneEditing #LipidNanoparticles #Biotechnology #TherapyDevelopment #GeneticEngineering #InnovativeMedicine #HealthcareTech

📊 Deformulation Assays for #CRISPR LNPs!?! Best for last? Our final Oligos, Oligos, Oligos! webinar will be held Nov 21st at 9 am EST / 15:00 CST. I know many industry teammates were just at the International mRNA Health Conference and are probably overloaded with new info, but you won’t want to miss this! Big thanks to all of our featured speakers in this November’s webinar series. I’ll admit I have a favorite; we saved the best for last! What are you to do as an analytical scientist working on a new multi cargo #LNP? You’re handed a formulated particle and need to start qualifying new methods for component concentrations, their integrity and more… Acuitas Therapeutics, Inc., known for breakthroughs in synthetic lipids and novel LNP formulations, has been working with us on assays of just this sort. We have a choose-your-own-adventure scenario to present: choose a novel size exclusion or rapid IP-RP method with some important method robustness tweaks. These are brilliant experimentation stories from 2 talented scientists: Mateusz Imiołek and Razvan Cojocaru! 🗓️ Register here: https://lnkd.in/e2CCvDgV #GeneTherapy #CellTherapy #Biotechnology #LifeatWaters #AnalyticalChemistry Waters Corporation ————————— I’m Matt Lauber - an analytical biochemist who loves separating biomolecules, detecting masses, and all things biotechnology! ☑️ Follow & subscribe 🔔

🔬 Virtual Screening: The Future of Drug Discovery Virtual screening is a cutting-edge in silico approach for modeling molecular interactions in target and lead discovery processes. It provides valuable data on ligand-target binding, offering key insights into the mechanisms of biomolecules involved in various diseases. Docking can be performed on a range of biomolecules, including proteins and nucleic acids, yielding critical information about different types of interactions. #VirtualScreening #Biotechnology #DrugDiscovery #BiomedicalResearch #MolecularInteractions #ProteinDocking #HealthScience #MedicineOfTheFuture

🚀 Exciting News: Introducing a new high-performance lyophilized product from New England Biolabs LyoSciences supporting molecular diagnostic developers! 🧬   Join us in revolutionizing nucleic acid amplification with our latest innovation: LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG)! https://lnkd.in/edcDg72A   Key Features: 🌿 Lyophilized format for ambient shipping and storage 🧬 One-step solution for Loop-Mediated Isothermal Amplification of DNA and RNA targets ⛔️ Thermolabile UDG and dUTP included to reduce carryover contamination 🌈 Includes fluorescent dye for real-time fluorescence measurement 🔥 WarmStart technology inhibits enzyme activity at room temperature   Whether you're in research, diagnostics, or biotech, our LyoPrime WarmStart fLAMP Mix offers unparalleled convenience and reliability. With a shelf life of 2 years at room temperature, you can trust in its performance whenever you need it.   Join the webinar on Lyophilizing Reagents to learn more about this groundbreaking technology and our expertise: https://lnkd.in/eTn4ZcEw   Let's amplify progress together! 💡 #NEB #NEBLS #Lyo #Lyophilisation #molecularbiology #DNA #RNA #diagnostics #biotech #LAMP

From the basics of cell culture to advanced 3D models and therapeutic applications, the quality and consistency of media and reagents are key! High-quality components ensure reliable and reproducible results, vital for research and clinical progress. By using specialized growth factors, cytokines, and cutting-edge live-cell analysis technologies like the Incucyte® system, researchers can optimize cell culture conditions, enhance data accuracy, and drive innovative treatments forward. Our latest eBook highlights the importance of meticulous cell culture practices, showing how superior media and technologies can lead to groundbreaking advancements in research and therapy. 📚✨ Download now: https://ow.ly/LvUl50Tv7i2 #CellCulture #CellAnalyis #3DCellCulture #Organoids #Spheroids #Biotechnology