Aspen Alert’s Post

Accelerating Cell Culture Media Development Using Bayesian Optimization-Based Iterative Experimental Design Here, we have applied a Bayesian Optimization-based iterative framework for experimental design to accelerate cell culture media development for two applications. First, we show this approach yields new compositions of media with cytokine supplementation to maintain the viability and distribution of PBMCs in culture. Second, we applied this framework to optimize the production of three recombinant proteins in K.phaffii cultivations. For both applications, we identified conditions with improved outcomes compared to the initial standard media using 3 to 30 times fewer experiments than other methods such as the Design of Experiments. Subsequently, we also demonstrated the extensibility of our approach to efficiently account for additional design factors through transfer learning. https://lnkd.in/g9c_7ZxK #aspenalert #biotech #bioprocess

"Had an incredible experience attending a 5-day hands-on workshop on Mammalian Cell Culture! Gained invaluable skills and insights into cell growth, maintenance, and analysis. Excited to apply these techniques in my future research and projects! #Biotechnology #CellCulture #HandsOnLearning"

Beyond Viability: Advancing CHO Cell Culture Process Strategies to Modulate Host Cell Protein Levels While purification strategies usually offer substantial HCP clearance, certain problematic HCPs, particularly lipases, pose significant challenges. This study investigates the accumulation of various problematic HCPs in CHO cell culture using transcriptomics, revealing correlations between cell culture parameters and HCP activity. Contrary to conventional assumptions, viability alone does not reliably predict HCP levels, with factors such as clone selection, host cell line choice, and media feed type significantly influencing HCP accumulation. Leveraging transcriptomics-based approaches, we demonstrate the potential of upstream process control strategies to mitigate HCP presence and improve biologic product quality. Our findings underscore the importance of considering diverse cell culture parameters in bioprocess optimization to ensure product stability and quality. https://lnkd.in/g27Z3pc3 #aspenalert #biotech #bioprocess

Welcome to Wednesday Words, where I will define one word used in biotechnology or biopharma each Wednesday morning! Cell culture media, also known as growth media, is an umbrella term that encompasses any gel or liquid created to support cellular growth in an artificial environment. When scientists remove cells, organs, or tissues from living creatures, they must keep them in an appropriate artificial environment. If you want to learn more, check out https://lnkd.in/gejwAP7d #wednesdaywords #biotechnology #biopharma

Our expertise in engineering cell-based assays to monitor protein degradation is industry-leading. Our cell line engineering team creates dTag cell lines for reliable target validation, Hibit cell lines for real-time monitoring of protein degradation, or HaloTag fusion proteins for dynamic degradation tracking. Explore the breadth of our tools for the cutting-edge discovery of protein degraders with precision at https://lnkd.in/dghrFMHX Our scientists engineer new cell-based assays to answer the most pressing questions in protein degrader discovery: -> Target validation > Target degradation tracking > Real-time monitoring of target degradation Want to learn more? Download our brochure at https://lnkd.in/diCvwZty #targetedproteindegradation #drugdiscovery #protac #proteindegradation #TPD #proteindegraders

Structure-based virtual screening has become an important tool to identify starting points for drug discovery https://lnkd.in/gppZhstb #Sciencedirect #Nature #Bioinformatics #BioNome #VirtualScreening #Mole culardynamicsimulation

𝐃𝐢𝐠𝐢𝐭𝐚𝐥 𝐏𝐂𝐑 𝐚𝐧𝐝 𝐑𝐞𝐚𝐥-𝐓𝐢𝐦𝐞 𝐏𝐂𝐑 ▶ 𝐆𝐞𝐭 𝐅𝐫𝐞𝐞 𝐀𝐜𝐜𝐞𝐬𝐬 𝐭𝐨 𝐃𝐞𝐦𝐨 𝐑𝐞𝐩𝐨𝐫𝐭, 𝐄𝐱𝐜𝐞𝐥 𝐏𝐢𝐯𝐨𝐭 𝐚𝐧𝐝 𝐓𝐨𝐂: https://lnkd.in/dqt_2Kbi Digital PCR is an end-point PCR method for absolute quantification and analyzing minority sequences against a backdrop of similar majority sequences. Digital PCR (dPCR) allows for the exact and sensitive measurement of nucleic acids. Real time PCR is a method for collecting data during the PCR process, integrating amplification and detection into a single phase. Nucleic acid quantification using real-time quantitative PCR is sensitive, specific, and repeatable. ▪ 𝐒𝐨𝐦𝐞 𝐨𝐟 𝐭𝐡𝐞 𝐃𝐢𝐠𝐢𝐭𝐚𝐥 𝐏𝐂𝐑 𝐚𝐧𝐝 𝐑𝐞𝐚𝐥-𝐓𝐢𝐦𝐞 𝐏𝐂𝐑 𝐌𝐚𝐫𝐤𝐞𝐭 𝐩𝐥𝐚𝐲𝐞𝐫𝐬 𝐚𝐫𝐞: ▪ Abbott ▪ Agilent Technologies. ▪ Analytik Jena ▪ Becton, Dickinson and Company ▪ Bio Molecular Systems ▪ Biomeme, Inc.. ▪ bioMérieux ▪ Bioneer ▪ Bio-Rad Laboratories. ▪ COLE-PARMER INSTRUMENT COMPANY LIMITED ▪ Danaher Corporation ▪ ELITechGroup - A Bruker Company ▪ Endress+Hauser Group ▪ Enzo Life Sciences, Inc. ▪ Eppendorf ▪ Roche. ▪ Fluidigm Sciences, Inc. ▪ JN Medsys Pte Ltd. ▪ Merck KGaA, Darmstadt, Germany ▪ Meridian Bioscience Inc., ▪ Promega Corporation ▪ QIAGEN ▪ QuidelOrtho ▪ Sacace Biotechnologies Srl ▪ Standard BioTools ▪ Stilla Technologies ▪ Takara Bio USA, Inc. ▪ Thermo Fisher Scientific. #DigitalPCR #RealTimePCR #NucleicAcidQuantification #PCRTechnology #DNAAnalysis #GeneticResearch #PCRMarket #Biotechnology

4️⃣ Today, we're excited to share insights into the crucial fourth step of our immunoassay development: Assay Setup and Optimization. 🔎 Once the foundation of core assay reagents is laid, our dedicated team springs into action to develop the assay in alignment with bioanalytical guidance documents. This stage is where precision and expertise converge to create assays of unparalleled accuracy and reliability. But what sets us apart? It's our groundbreaking AnySource® sample dilution technology that truly revolutionizes the game. With AnySource®, we have the flexibility to adapt assays to a myriad of matrices, whether it's animal, artificial, or human, as well as challenging matrices like CSF, synovial fluid, aqueous humor, and beyond. But we don't stop there. We're constantly pushing the boundaries to optimize every aspect of the assay. From fine-tuning required sample volumes for microsampling or rare matrix study support to enhancing selectivity and reducing background noise, we leave no stone unturned in our quest for perfection. Our unwavering commitment to excellence ensures that researchers and institutions can rely on our assays to deliver accurate and reproducible results, even in the most demanding of conditions. Join us as we continue to push the boundaries of science and empower researchers worldwide to make groundbreaking discoveries! #AssayDevelopment #Immunoassay #Science #Innovation #Research #Biotechnology #AnySource #SampleDilution #Optimization #Breakthroughs #Chimera #ChimeraBiotec